Both essential oils did show a weak estrogenic action in human breast cancer (MCF-7) cells (Henley et al 2007). ALDH1L1, in the presence of NADP+, oxidizes 10-formyltetrahydrofolate to tetrahydrofolate and CO2. As a potential drug carrier, it is essential to examine the safety and toxicity of citral incorporated into the NLC system to ensure its suitability for the future therapeutic usage of the formulation.
Neral's lemon odor is less intense, but sweeter.

Free amount of citral In brief, upon mice sacrifice, the spleen collected was washed with 5 mL of PBS. Eventually, the increase is AST level caused an elevation in the level of ALT (Giannini, Testa & Savarino, 2005).

Citral is different from other terpenoids in that it is actually a pair of isomers connected by a double bond; geranial (citral A) and neral (citral B). Citracal + D is used to treat or prevent a calcium deficiency.

In fact, citronella has been registered as a gentle, plant-based insect repellent in the United States since 1948. The experiment was conducted according to the protocol provided with the kit.

(+)-Limonene had a quenching effect on cinnamaldehyde sensitization in 3 of 11 human subjects, and eugenol had a similar effect in 7 of the same 11 cinnamaldehyde-sensitive subjects. Temesvári et al (2002) found that, of 104 FM-negative patients, 18 (11.9%) subsequently tested positive to one or more of six of the eight constituents. The concentration of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and creatinine level in mice serum were analyzed accordingly using Hitachi automatic analyzer (Hitachi-902, LTD, JAPAN).

Two studies showed 1–1.7% of people to be allergic to citral, with allergies frequently reported. The IFRA Standard restricts the use of citral in fragrances because of potential sensitization and recommends that citral only be used in association with substances that prevent a sensitizing effect.

Together, these versatile characteristics of citral have attracted researchers to further exploit its increased efficiency. Thimerosal, D-Sorbitol, Tween-80, citral 95%, Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), Thiazolyl Blue Tetrazolium Bromide were all purchased from Sigma-Aldrich (St. Louis, MO, USA). EWG provides information on personal care product ingredients from the published scientific literature, to supplement incomplete data available from companies and the government.

It also has strong antimicrobial qualities,[5] and pheromonal effects in acari and insects.[6][7]. Next, the splenocytes were seeded at a density of 2 × 106 cells/mL in a 96-well plate. The incorporation of a water insoluble anticancer compound (Amoitone B) with NLC was reported to enhance its bioavailability (Luan et al., 2013). An earlier report showed an endothelium-independent vasorelaxation by citral (main component in C. citratus) via the blockage of Ca2+ influx, while the methanolic extracts of the leaves, roots, and stems displayed vasorelaxation activities via the prostacyclin (PGI2) channel (Devi et al., 2012).

It is apparent that the Wistar rat ventral prostate is reactive to citral. Thus, citral may be used as a preservative in the formulation of cosmetics and personal care products.
Citral, applied in ethanol at a dose of 185 mg/kg/day to the shaved dorsal skin of male Wistar rats for three months, produced benign prostatic hyperplasia (BPH; Abramovici et al 1985). Viability

In this formulation of NLC, Citral conveyed 98.9 ± 0.124% of entrapment efficiency in the system with 9.84 ± 0.041% of drug loading capacity in the lipids concentration. × For aqueous phase, D-Sorbitol (4.75% [w/v]), Thimerosal (0.005% [w/v]), and 1% Tween-80 were dissolved in double-distilled water at 70 °C and was then added into the lipid phase under constant stirring for 5 min. Mas Jaffri Masarudin analyzed the data, reviewed drafts of the paper. The supernatant from the splenocytes was mixed with Griess Reagent and incubated for 30 min before being measured at 548 nm using microplate reader (Beckman Coulter, USA).

Citral is one of the active components of citronella oil, which is a common ingredient used in insect repellents and citronella candles.